Phospho-SHIP (Y1020) | HUABIO ET1701-5 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-SHIP (Y1020)

catalog :

ET1701-5

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JJ082-03

reactivity :

human

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

ICC staining of Phospho-SHIP (Y1020) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 2 :

ICC staining of Phospho-SHIP (Y1020) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

ICC staining of Phospho-SHIP (Y1020) in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

ET1701-5

Target name :

Phospho-SHIP (Y1020)

Species reactivity :

Human

Applications :

WB,IF-Cell,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Tyr1020 of Human SHIP aa 991-1040 / 1,189.

Uniprot id :

Q92835>SwissProt: Q92835 Human

Host :

Rabbit

Clone number :

JJ082-03

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A549, Hela, HepG2.

Molecular wt :

Predicted band size: 133 kDa

Subcellular location :

Cell membrane, Cytoplasm, Cytoskeleton, Membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IP: Use at an assay dependent concentration.

more info or order :

HUABIO product webpage