JNK1+JNK3 | HUABIO ET1701-47 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

JNK1+JNK3

catalog :

ET1701-47

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JJ08-86

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of JNK1+JNK3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: PC-12 cell lysate

image 2 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-JNK1+JNK3 antibody (ET1701-47) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-47) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1701-47

Target name :

JNK1+JNK3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human JNK1 + JNK3 aa 1-423 / 427.

Uniprot id :

P45983>SwissProt: P45983 Human;SwissProt: P53779 Human;SwissProt: Q61831 Mouse;SwissProt: Q91Y86 Mouse;SwissProt: P49185 Rat;SwissProt: P49187 Rat

Host :

Rabbit

Clone number :

JJ08-86

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

NIH/3T3 cell lysate, PC-12 cell lysate, human brain tissue.

Molecular wt :

Predicted band size: 48/53 kDa

Subcellular location :

Cytoplasm, Nucleus, Membrane, Mitochondrion.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IP: Use at an assay dependent concentration. ;IHC-P: 1:200-1:1,000

more info or order :

HUABIO product webpage