Phospho-c-Jun (T91) | HUABIO ET1701-32 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-c-Jun (T91)

catalog :

ET1701-32

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JJ080-9

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-c-Jun (T91) on different lysates with Rabbit anti-Phospho-c-Jun (T91) antibody (ET1701-32) at 1/500 dilution. Lane 1: A549 whole cell lysate (20 µg/Lane) Lane 2: A549 treated with 250ng/mL anisomycin for 30 minutes whole cell lysate (20 µg/Lane) Lane 3: NIH/3T3 whole cell lysate (20 µg/Lane) Lane 4: NIH/3T3 treated with 250ng/mL anisomycin for 30 minutes whole cell lysate (20 µg/Lane) Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-32) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Phospho-c-Jun (T91) on different lysates with Rabbit anti-Phospho-c-Jun (T91) antibody (ET1701-32) at 1/500 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with UV for 15 minutes then recover 30 minutes whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-32) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of A549 cells untreated / treated with 250ng/mL anisomycin for 30 minutes labeling Phospho-c-Jun (T91) with Rabbit anti-Phospho-c-Jun (T91) antibody (ET1701-32) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-c-Jun (T91) antibody (ET1701-32) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

ET1701-32

Target name :

Phospho-c-Jun (T91)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Thr91 of Human c-Jun aa 60-109 / 331.

Uniprot id :

P05412>SwissProt: P05412 Human;SwissProt: P05627 Mouse;SwissProt: P17325 Rat

Host :

Rabbit

Clone number :

JJ080-9

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A549 treated with 250ng/mL anisomycin for 30 minutes whole cell lysate, NIH/3T3 treated with 250ng/mL anisomycin for 30 minutes whole cell lysate, NIH/3T3 treated with UV for 15 minutes then recover 30 minutes whole cell lysate, A549 cells treated with 250ng/mL anisomycin for 30 minutes, NIH/3T3 cells treated with UV for 15 minutes then recover 30 minutes, human breast carcinoma tissue, human skin tissue, human breast tissue.

Molecular wt :

Predicted band size: 36 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500 ;IF-Cell: 1:500-1:5,000 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1701-32_4.jpg

Pic legend4 :

Immunocytochemistry analysis of NIH/3T3 cells untreated / treated with UV for 15 minutes then recover 30 minutes labeling Phospho-c-Jun (T91) with Rabbit anti-Phospho-c-Jun (T91) antibody (ET1701-32) at 1/5,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-c-Jun (T91) antibody (ET1701-32) at 1/5,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1701-32_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun (T91) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-32, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1701-32_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Phospho-c-Jun (T91) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-32, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1701-32_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Phospho-c-Jun (T91) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-32, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage