product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
SIRT6
catalog :
ET1701-29
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JJ20-69
reactivity :
human
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of SIRT6 on different lysates with Rabbit anti-SIRT6 antibody (ET1701-29) at 1/500 dilution.
Lane 1: HeLa cell lysate
Lane 2: MDA-MB-231 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 2 minutes 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-29) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of SIRT6 on different lysates with Rabbit anti-SIRT6 antibody (ET1701-29) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-SIRT6 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 62 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-29) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
ET1701-29
Target name :
SIRT6
Species reactivity :
Human
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human SIRT6 aa 306-355 / 355.
Uniprot id :
Q8N6T7>SwissProt: Q8N6T7 Human
Host :
Rabbit
Clone number :
JJ20-69
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, MDA-MB-231 cell lysate.
Molecular wt :
Predicted band size: 39 kDa
Subcellular location :
Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
Advanced Validation :
Knockdown (KD)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
related products
browse more products
questions and comments