product summary
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company name :
HUABIO
product type :
antibody
product name :
Phospho-GluR1 (S845)
catalog :
ET1701-28
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JJ2009
reactivity :
human, mouse, rat
application :
western blot, flow cytometry
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO ET1701-28 image 1
Western blot analysis of Phospho-GluR1 (S845) on different lysates with Rabbit anti-Phospho-GluR1 (S845) antibody (ET1701-28) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour Lane 4: Rat brain tissue lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 40 µg/Lane. Predicted band size: 102 kDa Observed band size: 102 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO ET1701-28 image 2
Flow cytometric analysis of Phospho-GluR1 (S845) was done on N2A cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-28, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
ET1701-28
Target name :
Phospho-GluR1 (S845)
Species reactivity :
Mouse,Rat,Human
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser845 of Human GluR1 aa 831-880 / 906.
Uniprot id :
P42261>SwissProt: P42261 Human;SwissProt: P23818 Mouse;SwissProt: P19490 Rat
Host :
Rabbit
Clone number :
JJ2009
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Mouse brain tissue lysate, rat brain tissue lysate, mouse brain tissue lysate the membrane treated with λpp for 1 hour, rat brain tissue lysate the membrane treated with λpp for 1 hour, N2A.
Molecular wt :
Predicted band size: 102 kDa
Subcellular location :
Cell membrane, Endoplasmic reticulum membrane, Cell junction, Cell projection.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000 ;FC: 1:10-1:50
Advanced Validation :
Cell treatment (CT)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!