Raf1 | HUABIO ET1701-21 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Raf1

catalog :

ET1701-21

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JJ086-06

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Raf1 on different lysates with Rabbit anti-Raf1 antibody (ET1701-21) at 1/1,000 dilution. Lane 1: A375 cell lysate Lane 2: HepG2 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 73 kDa Observed band size: 73 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Raf1 on different lysates with Rabbit anti-Raf1 antibody (ET1701-21) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD Raf1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 73 kDa Observed band size: 73 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-21) at 1/1,000 dilution was used in 2% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1701-21

Target name :

Raf1

Species reactivity :

Human,Mouse,Rat

Applications :

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Raf1 aa 1-50/648.

Uniprot id :

P04049>SwissProt: P04049 Human;SwissProt: Q99N57 Mouse;SwissProt: P11345 Rat

Host :

Rabbit

Clone number :

JJ086-06

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A375 cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, A549 cell lysate.

Molecular wt :

Predicted band size: 73 kDa

Subcellular location :

Cytoplasm, Cell membrane, Mitochondrion, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage