MEKK3 | HUABIO ET1612-98 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MEKK3

catalog :

ET1612-98

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SD0839

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MEKK3 on different lysates with Rabbit anti-MEKK3 antibody (ET1612-98) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si MEKK3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-98) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of MEKK3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: A431 cell lysate

image 3 :

ICC staining of MEKK3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-98, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

ET1612-98

Target name :

MEKK3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human MEKK3 aa 1-50 / 626.

Uniprot id :

Q99759>SwissProt: Q99759 Human;SwissProt: Q61084 Mouse;Unigene: 72680 Rat

Host :

Rabbit

Clone number :

SD0839

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293T cell lysate, A431 cell lysate, Hela, MCF-7, SKOV-3, mouse uterus tissue, rat uterus tissue.

Molecular wt :

Predicted band size: 71 kDa

Subcellular location :

Cytosol, cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1612-98_4.jpg

Pic legend4 :

ICC staining of MEKK3 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-98, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1612-98_5.jpg

Pic legend5 :

ICC staining of MEKK3 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-98, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1612-98_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-MEKK3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1612-98_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-MEKK3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1612-98_8.jpg

Pic legend8 :

Flow cytometric analysis of MEKK3 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-98, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage