14-3-3 Theta | HUABIO ET1612-97 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

14-3-3 Theta

catalog :

ET1612-97

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SD084-02

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of 14-3-3 Theta on different lysates with Rabbit anti-14-3-3 Theta antibody (ET1612-97) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-14-3-3 Theta KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-97) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of 14-3-3 Theta on different lysates with Rabbit anti-14-3-3 Theta antibody (ET1612-97) at 1/1,000 dilution. Lane 1: Rat testis tissue lysate Lane 2: Rat brain tissue lysate Lane 3: C6 cell lysate Lane 4: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-97) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining of 14-3-3 Theta in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-97, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

ET1612-97

Target name :

14-3-3 Theta

Species reactivity :

Human,Rat,Mouse

Applications :

WB,IF-Cell,IF-Tissue,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human 14-3-3 Theta aa 1-50 / 245.

Uniprot id :

P27348>SwissProt: P27348 Human;SwissProt: P68254 Mouse;SwissProt: P68255 Rat

Host :

Rabbit

Clone number :

SD084-02

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Rat testis tissue lysate, rat brain tissue lysate, C6 cell lysate, MCF7 cell lysate, Hela, A431, A549, SH-SY5Y.

Molecular wt :

Predicted band size: 28 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:5,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;FC: 1:50-1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1612-97_4.jpg

Pic legend4 :

ICC staining of 14-3-3 Theta in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-97, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1612-97_5.jpg

Pic legend5 :

ICC staining of 14-3-3 Theta in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-97, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1612-97_6.jpg

Pic legend6 :

Flow cytometric analysis of 14-3-3 Theta was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-97, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage