HDAC8 | HUABIO ET1612-90 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

HDAC8

catalog :

ET1612-90

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SD082-07

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of HDAC8 on different lysates with Rabbit anti-HDAC8 antibody (ET1612-90) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-90) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of HDAC8 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

ET1612-90

Target name :

HDAC8

Species reactivity :

Human,Mouse,Rat

Applications :

WB,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human HDAC8 aa 61-110 / 377.

Uniprot id :

Q9BY41>SwissProt: Q9BY41 Human;SwissProt: Q8VH37 Mouse;SwissProt: B1WC68 Rat

Host :

Rabbit

Clone number :

SD082-07

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

K562 cell lysate, Hela cell lysate, K562.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Nucleus, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

more info or order :

HUABIO product webpage