14-3-3 gamma | HUABIO ET1612-9 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

14-3-3 gamma

catalog :

ET1612-9

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SD20-65

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of 14-3-3 gamma on different lysates with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-14-3-3 gamma KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-9) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of 14-3-3 gamma on different lysates with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-9) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of 14-3-3 gamma on different lysates with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/500 dilution. Lane 1: A431 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-9) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1612-9

Target name :

14-3-3 gamma

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human 14-3-3 gamma aa 131-180 / 247.

Uniprot id :

P61981>SwissProt: P61981 Human;SwissProt: P61982 Mouse;SwissProt: P61983 Rat

Host :

Rabbit

Clone number :

SD20-65

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293T cell lysate, K562 cell lysate, A431 cell lysate, Hela cell lysate, K562, human breast cancer tissue, human lung cancer tissue, human ovary cancer tissue.

Molecular wt :

Predicted band size: 28 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:200-1:1,000 ;FC: 1:10-1:50

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1612-9_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-9) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1612-9_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-9) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1612-9_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-9) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1612-9_7.jpg

Pic legend7 :

Flow cytometric analysis of 14-3-3 gamma was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-9, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage