Phospho-CDC37 (S13) | HUABIO ET1612-74 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-CDC37 (S13)

catalog :

ET1612-74

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SD08-50

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-CDC37 (S13) on different lysates with Rabbit anti-Phospho-CDC37 (S13) antibody (ET1612-74) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: C2C12 cell lysate Lane 4: PC-12 cell lysate Lane 5: HEK-293 cell lysate, treated with λpp for 1 hour Lane 6: C2C12 cell lysate, treated with λpp for 1 hour Lysates/proteins at 15 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-74) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1612-74

Target name :

Phospho-CDC37 (S13)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser13 of human CDC37.

Uniprot id :

Q16543>SwissProt: Q16543 Human;SwissProt: Q61081 Mouse;SwissProt: Q63692 Rat

Host :

Rabbit

Clone number :

SD08-50

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HEK-293 cell lysate, HeLa cell lysate, C2C12 cell lysate, PC-12 cell lysate, HEK-293 cell lysate treated with λpp for 1 hour, C2C12 cell lysate treated with λpp for 1 hour.

Molecular wt :

Predicted band size: 45 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage