PAX6 | HUABIO ET1612-58 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PAX6

catalog :

ET1612-58

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SD081-03

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PAX6 on different lysates with Rabbit anti-PAX6 antibody (ET1612-58) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate (negative) Lane 3: 293T cell lysate Lane 4: RAW264.7 cell lysate Lane 5: Mouse cerebellum tissue lysate Lane 6: Rat cerebellum tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: Lane 1-3: 1 minute; Lane 4-6: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-58) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Fluorescence multiplex immunohistochemical analysis of mouse brain (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-NeuN (ET1602-12, red), anti-PAX6 (ET1612-58, green), anti-CD34 (ET1606-11, gray), anti-MAP2 (HA500177, magenta) and anti-TBR1 (ET1702-97, yellow) on mouse brain. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in five rounds of staining: in the order of ET1602-12 (1/5,000 dilution), ET1612-58 (1/1,000 dilution), ET1606-11 (1/2,000 dilution), HA500177 (1/5,000 dilution) and ET1702-97 (1/1,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

image 3 :

Fluorescence multiplex immunohistochemical analysis of mouse pancreas (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-Cytokeratin 19 (ET1601-6, White) and anti-PAX6 (ET1612-58, Violet) on pancreas. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in two rounds of staining: in the order of ET1601-6 (1/5,000 dilution) and ET1612-58 (1/1,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.

product information

SKU :

ET1612-58

Target name :

PAX6

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC,mIHC,IF-Cell,IF-Tissue,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human PAX6 aa 373-422 / 422.

Uniprot id :

P26367>SwissProt: P26367 Human;SwissProt: P63015 Mouse;SwissProt: P63016 Rat

Host :

Rabbit

Clone number :

SD081-03

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, 293T cell lysate, RAW264.7 cell lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate, human pancreas tissue, mouse cerebellum tissue, mouse pancreas tissue, rat pancreas tissue, mouse eyeball tissue, rat eyeball tissue, rat cerebellum tissue, RAW264.7.

Molecular wt :

Predicted band size: 47 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;FC: 1:1,000 ;IHC-P: 1:200-1:2,000 ;mIHC: 1:1,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:800 ;IHC-Fr: 1:500

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1612-58_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PAX6 antibody (ET1612-58) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-58) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1612-58_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-PAX6 antibody (ET1612-58) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-58) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1612-58_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-PAX6 antibody (ET1612-58) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-58) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1612-58_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-PAX6 antibody (ET1612-58) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-58) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1612-58_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue with Rabbit anti-PAX6 antibody (ET1612-58) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1612-58_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat eyeball tissue using anti-PAX6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-58, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1612-58_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-PAX6 antibody (ET1612-58) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET1612-58_11.jpg

Pic legend11 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/ET1612-58_12.jpg

Pic legend12 :

Immunocytochemistry analysis of RAW264.7 cells labeling PAX6 with Rabbit anti-PAX6 antibody (ET1612-58) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PAX6 antibody (ET1612-58) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img13 :

https://storage.huabio.cn/huabio/productImg/ET1612-58_13.jpg

Pic legend13 :

Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1/800

Pic img14 :

https://storage.huabio.cn/huabio/productImg/ET1612-58_14.jpg

Pic legend14 :

Flow cytometric analysis of RAW264.7 cells labeling PAX6. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1612-58, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage