Phospho-MEK1 (T292) | HUABIO ET1612-42 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-MEK1 (T292)

catalog :

ET1612-42

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SD2088

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-MEK1 (T292) on different lysates with Rabbit anti-Phospho-MEK1 (T292) antibody (ET1612-42) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate Lane 3: PC-12 cell lysate Lane 4: PC-12 treated with 100ng/mL Nocodazole for 18 hours cell lysate Lane 5: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate, then the membrane treated with λpp for 1 hour Lane 6: PC-12 treated with 100ng/mL Nocodazole for 18 hours cell lysate, then the membrane treated with λpp for 1 hour Lane 7: NIH/3T3 cell lysate Lane 8: NIH/3T3 treated with 100ng/mL Nocodazole for 18 hours cell lysate Lane 9: NIH/3T3 treated with 100ng/mL Nocodazole for 18 hours cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-MEK1 (T292) antibody (ET1612-42) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-42) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1612-42

Target name :

Phospho-MEK1 (T292)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Thr292 of Human MEK1 aa 271-320 / 393.

Uniprot id :

Q02750>SwissProt: Q02750 Human;SwissProt: P31938 Mouse;SwissProt: Q01986 Rat

Host :

Rabbit

Clone number :

SD2088

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate, PC-12 cell lysate, PC-12 treated with 100ng/mL Nocodazole for 18 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 100ng/mL Nocodazole for 18 hours cell lysate, mouse brain tissue.

Molecular wt :

Predicted band size: 43 kDa

Subcellular location :

Cytoplasm, Nucleus, Membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:200

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage