14-3-3 sigma | HUABIO ET1612-41 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

14-3-3 sigma

catalog :

ET1612-41

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SD2070

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of 14-3-3 sigma on A431 cell lysates with Rabbit anti-14-3-3 sigma antibody (ET1612-41) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 1 minute 11 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-41) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-14-3-3 sigma antibody (ET1612-41) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-41) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-14-3-3 sigma antibody (ET1612-41) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-41) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1612-41

Target name :

14-3-3 sigma

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human 14-3-3 sigmaaa 50-99 / 248.

Uniprot id :

P31947>SwissProt: P31947 Human;SwissProt: O70456 Mouse;Entrez Gene: 313017 Rat

Host :

Rabbit

Clone number :

SD2070

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysates, human skin tissue, mouse skin tissue, rat skin tissue.

Molecular wt :

Predicted band size: 28 kDa

Subcellular location :

Cytoplasm, Nucleus, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:2,000-1:10,000 ;IF-Tissue: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1612-41_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-14-3-3 sigma antibody (ET1612-41) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-41) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1612-41_5.jpg

Pic legend5 :

Immunofluorescence analysis of paraffin-embedded human skin tissue labeling 14-3-3 sigma with Rabbit anti-14-3-3 sigma antibody (ET1612-41) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1612-41, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage