product summary
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company name :
HUABIO
product type :
antibody
product name :
Caspase-5
catalog :
ET1612-29
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
SD203-2
reactivity :
human
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Caspase-5 on different lysates with Rabbit anti-Caspase-5 antibody (ET1612-29) at 1/5,000 dilution.
Lane 1: THP-1 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: SHG-44 cell lysate (20 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-29) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Caspase-5 antibody (ET1612-29) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-29) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
ET1612-29
Target name :
Caspase-5
Species reactivity :
Human
Applications :
WB,IF-Tissue,IHC-P,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human Caspase 5 aa 1-50 / 434.
Uniprot id :
P51878>SwissProt: P51878 Human
Host :
Rabbit
Clone number :
SD203-2
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
THP-1 cell lysate, HeLa cell lysate, A549 cell lysate, SHG-44 cell lysate, human liver tissue.
Molecular wt :
Predicted band size: 50 kDa
Subcellular location :
NLRP1 inflammasome complex, cytoplasm.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000
;IF-Tissue: 1:50
;IHC-P: 1:50
;IP: Use at an assay dependent concentration.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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