pro Caspase-7 | HUABIO ET1612-28 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

pro Caspase-7

catalog :

ET1612-28

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SD20-57

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of pro Caspase-7 on human lung lysates with Rabbit anti-pro Caspase-7 antibody (ET1612-28) at 1/1000 dilution. Lysates at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 35 kDa Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-28) at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining pro Caspase 7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining pro Caspase 7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ET1612-28

Target name :

pro Caspase-7

Species reactivity :

Human

Applications :

WB,IF-Cell,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Caspase 7 aa 1-50 / 303.

Uniprot id :

P55210>SwissProt: P55210 Human

Host :

Rabbit

Clone number :

SD20-57

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Hela, HepG2, human liver tissue, human kidney tissue,human lung lysates.

Molecular wt :

34 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1612-28_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-pro Caspase 7 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1612-28_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-pro Caspase 7 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage