Cyclin B2 | HUABIO ET1612-21 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cyclin B2

catalog :

ET1612-21

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SD2045

reactivity :

human, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Cyclin B2 on different lysates with Rabbit anti-Cyclin B2 antibody (ET1612-21) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD Cyclin B2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Cyclin B2 on different lysates with Rabbit anti-Cyclin B2 antibody (ET1612-21) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: PC-12 cell lysate (20 µg/Lane) Lane 3: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining of Cyclin B2 in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-21, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

ET1612-21

Target name :

Cyclin B2

Species reactivity :

Human,Rat

Applications :

WB,IF-Cell,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Cyclin B2 aa 1-102 / 398.

Uniprot id :

O95067>SwissProt: O95067 Human;Entrez Gene: 363088 Rat

Host :

Rabbit

Clone number :

SD2045

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A549 cell lysate, PC-12 cell lysate, rat testis tissue lysate, PC-12.

Molecular wt :

Predicted band size: 45 kDa

Subcellular location :

Centrosome, microtubule cytoskeleton, cytosol, nucleus, cyclin-dependent protein kinase holoenzyme complex, cytoplasm, host cell nucleus, membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage