Insulin Receptor Beta | HUABIO ET1611-90 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Insulin Receptor Beta

catalog :

ET1611-90

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN20-13

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Insulin Receptor Beta on different lysates with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/1,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si Insulin Receptor Beta cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 156 kDa Observed band size: 95 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-90) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Insulin Receptor Beta on different lysates with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: T-47D cell lysate (low expression) (20 µg/Lane) Lane 4: Hepa1-6 cell lysate (20 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 156 kDa Observed band size: 95/45 kDa Exposure time: Lane 1-3: 1 minute 2 seconds; Lane 4-5: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-90) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of Insulin Receptor Beta on mouse skeletal muscle tissue lysates with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 156 kDa Observed band size: 95 kDa Exposure time: 3 minutes; 4~20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-90) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1611-90

Target name :

Insulin Receptor Beta

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Insulin receptor aa 930-970.

Uniprot id :

P06213>SwissProt: P06213 Human;SwissProt: P15208 Mouse;SwissProt: P15127 Rat

Host :

Rabbit

Clone number :

SN20-13

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HCT 116 cell lysate, MDA-MB-231 cell lysate, HepG2 cell lysate, T-47D cell lysate, Rat liver tissue lysate, mouse skeletal muscle tissue lysates, HepG2, LO2, RH-35, rat liver tissue, mouse liver tissue, human liver tissue.

Molecular wt :

Predicted band size: 156 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC: 1:1,000

Advanced Validation :

Knockdown (KD),Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1611-90_4.jpg

Pic legend4 :

ICC staining of Insulin Receptor Beta in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-90, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1611-90_5.jpg

Pic legend5 :

ICC staining of Insulin Receptor Beta in LO2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-90, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1611-90_6.jpg

Pic legend6 :

ICC staining of Insulin Receptor Beta in RH-35 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-90, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1611-90_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-90) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1611-90_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-90) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1611-90_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-90) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage