BRG1 | HUABIO ET1611-85-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

BRG1

catalog :

ET1611-85-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN20-03

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: E14.5 embryo Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Mouse Site: E14.5 embryo Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required

image 3 :

Application: IHC-Fr Species: Mouse Site: E14.5 embryo Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

product information

SKU :

ET1611-85-50UL

Target name :

BRG1

Species reactivity :

Human,Mouse,Rat,Pig

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IHC-Fr,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human BRG1 aa 240-280.

Uniprot id :

P51532>SwissProt: P51532 Human;SwissProt: Q3TKT4 Mouse;SwissProt: Q8K1P7 Rat

Host :

Rabbit

Clone number :

SN20-03

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa, HeLa cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, HepG2, NIH/3T3, human tonsil tissue, human kidney tissue, human breast carcinoma tissue, mouse testis tissue, mouse colon tissue, mouse kidney tissue, mouse epididymis tissue, rat colon tissue, rat kidney tissue, rat brain tissue, mouse hippocampus tissue, mouse cerebral cortex tissue.

Molecular wt :

Predicted band size: 185 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:20,000-1:50,000 ;IF-Cell: 1:200-1:1,000 ;IHC-P: 1:1,000-1:5,000 ;IF-Tissue: 1:200-1:500 ;IHC-Fr: 1:1,000 ;FC: 1:1,000

Advanced Validation :

Relative expression (RE),Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_4.jpg

Pic legend4 :

Application: IHC-Fr Species: Mouse Site: Testis Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_5.jpg

Pic legend5 :

Western blot analysis of BRG1 on different lysates with Rabbit anti-BRG1 antibody (ET1611-85) at 1/50,000 dilution and competitor's antibody at 1/10,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: A549 cell lysate (negative) Lane 4: NIH/3T3 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 185 kDa Observed band size: 200 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-85) at 1/50,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_6.jpg

Pic legend6 :

Immunocytochemistry analysis of HeLa cells labeling BRG1 with Rabbit anti-BRG1 antibody (ET1611-85) at 1/1,000 dilution and competitor's antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BRG1 antibody (ET1611-85) at 1/1,000 dilution and competitor's antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_7.jpg

Pic legend7 :

Western blot analysis of BRG1 on different lysates with Rabbit anti-BRG1 antibody (ET1611-85) at 1/2,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si BRG1 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 185 kDa Observed band size: 200 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-85) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_8.jpg

Pic legend8 :

Immunocytochemistry analysis of HepG2 cells labeling BRG1 with Rabbit anti-BRG1 antibody (ET1611-85) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BRG1 antibody (ET1611-85) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_9.jpg

Pic legend9 :

Immunocytochemistry analysis of NIH/3T3 cells labeling BRG1 with Rabbit anti-BRG1 antibody (ET1611-85) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BRG1 antibody (ET1611-85) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-BRG1 antibody (ET1611-85) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-85) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-BRG1 antibody (ET1611-85) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-85) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_12.jpg

Pic legend12 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-BRG1 antibody (ET1611-85) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-85) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img13 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_13.jpg

Pic legend13 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-BRG1 antibody (ET1611-85) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-85) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img14 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_14.jpg

Pic legend14 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-BRG1 antibody (ET1611-85) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-85) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img15 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_15.jpg

Pic legend15 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-BRG1 antibody (ET1611-85) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-85) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img16 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_16.jpg

Pic legend16 :

Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue with Rabbit anti-BRG1 antibody (ET1611-85) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-85) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img17 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_17.jpg

Pic legend17 :

Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-BRG1 antibody (ET1611-85) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-85) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img18 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_18.jpg

Pic legend18 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-BRG1 antibody (ET1611-85) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-85) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img19 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_19.jpg

Pic legend19 :

Application: IF-tissue Species: Rat Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1:200

Pic img20 :

https://storage.huabio.cn/huabio/productImg/ET1611-85_20.jpg

Pic legend20 :

Flow cytometric analysis of HeLa cells labeling BRG1. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1611-85, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage