Phospho-PKC zeta (T560) | HUABIO ET1611-78 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-PKC zeta (T560)

catalog :

ET1611-78

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN0753

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-PKC zeta (T560) on different lysates with Rabbit anti-Phospho-PKC zeta (T560) antibody (ET1611-78) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate (20 µg/Lane) Lane 2: Mouse eyeball tissue lysate (30 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate, the membrane treated with λpp for 1 hour (20 µg/Lane) Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-78) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Phospho-PKC zeta (T560) antibody (ET1611-78) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-78) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Phospho-PKC zeta (T560) antibody (ET1611-78) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-78) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1611-78

Target name :

Phospho-PKC zeta (T560)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Thr560 of Human PKC zeta aa 531-580 / 592.

Uniprot id :

Q05513>SwissProt: Q05513 Human;SwissProt: Q02956 Mouse;SwissProt: P09217 Rat

Host :

Rabbit

Clone number :

SN0753

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SK-Br-3 cell lysate, Mouse eyeball tissue lysate, C6 cell lysate, human testis tissue, mouse testis tissue, rat testis tissue.

Molecular wt :

Predicted band size: 68 kDa

Subcellular location :

Cell junction, Cytoplasm, Endosome, Membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:50

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1611-78_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Phospho-PKC zeta (T560) antibody (ET1611-78) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-78) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage