FANCD2 | HUABIO ET1611-67 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

FANCD2

catalog :

ET1611-67

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN73-08

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of FANCD2 on different lysates with Rabbit anti-FANCD2 antibody (ET1611-67) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse testis tissue lysate Lane 6: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 164 kDa Observed band size: 164 kDa Exposure time: Lane 1: 8 seconds; Lane 2-3: 30 seconds; Lane 4-6: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-67) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells labeling FANCD2 with Rabbit anti-FANCD2 antibody (ET1611-67) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FANCD2 antibody (ET1611-67) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of C6 cells labeling FANCD2 with Rabbit anti-FANCD2 antibody (ET1611-67) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FANCD2 antibody (ET1611-67) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

ET1611-67

Target name :

FANCD2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human FANCD2 aa 191-240 / 1,451.

Uniprot id :

Q9BXW9>SwissProt: Q9BXW9 Human;SwissProt: Q80V62 Mouse;SwissProt: Q6IV68 Rat

Host :

Rabbit

Clone number :

SN73-08

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, K-562 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, HeLa, C6, mouse testis tissue, rat stomach tissue, rat testis tissue, mouse stomach tissue.

Molecular wt :

Predicted band size: 164 kDa

Subcellular location :

Nucleus, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:100 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1611-67_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-FANCD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1611-67_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-FANCD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1611-67_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-FANCD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1611-67_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-FANCD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage