CD13 | HUABIO ET1611-61 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD13

catalog :

ET1611-61

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN71-04

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (ET1611-61) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CD13 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 110 kDa Observed band size: 150 kDa Exposure time: 110 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-61) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (ET1611-61) at 1/2,000 dilution. Lane 1: 293T cell lysate (negative) (20 µg/Lane) Lane 2: PANC-1 cell lysate (5 µg/Lane) Lane 3: Mouse kidney tissue lysate (20 µg/Lane) Lane 4: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 110 kDa Observed band size: 150 kDa Exposure time: 45 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-61) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD13 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1611-61

Target name :

CD13

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CD13 aa 401-450 / 967.

Uniprot id :

P15144>SwissProt: P15144 Human;SwissProt: P97449 Mouse;SwissProt: P15684 Rat

Host :

Rabbit

Clone number :

SN71-04

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

PANC-1 cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate, human tonsil tissue, human liver tissue, human breast tissue, human kidney tissue, mouse pancreas tissue.

Molecular wt :

Predicted band size: 110 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:100-1:500 ;IP: 1-2μg/sample

Advanced Validation :

Knockdown (KD),Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1611-61_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD13 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1611-61_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-CD13 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1611-61_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD13 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1611-61_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-CD13 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1611-61_8.jpg

Pic legend8 :

CD13 was immunoprecipitated from 0.2 mg PANC-1 cell lysate with ET1611-61 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1611-61 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: PANC-1 cell lysate (input) Lane 2: ET1611-61 IP in PANC-1 cell lysate Lane 3: Rabbit IgG instead of ET1611-61 in PANC-1 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 20 seconds; ECL: K1801

more info or order :

HUABIO product webpage