antibody

Anti-ACE2 Antibody [SN0754]

ET1611-58TR

20 uL

99 USD

monoclonal

domestic rabbit

nonconjugated

SN0754

Syrian golden hamster, human, mouse, rat

western blot, immunoprecipitation, immunohistochemistry - paraffin section

Anti-ACE2 Antibody [SN0754]

Human,Mouse,Hamster,Rat

WB,IF-Cell,IHC-P,IP

Non-conjugated

Synthetic peptide within Human ACE2 aa 181-230 / 805.

SwissProt: Q9BYF1 Human;SwissProt: Q8R0I0 Mouse;SwissProt: Q5EGZ1 Rat;SwissProt: A0A1U7QTA1 Hamster

Rabbit

SN0754

IgG

20 uL

99 USD

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Liquid

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Protein A affinity purified.

Recombinant Rabbit monoclonal Antibody

Human kidney tissue lysate, human small intestine tissue lysate, Hamster testis lysates, Hamster stomach tissue lysates, mouse kidney tissue lysate, mouse testis tissue lysate, 293, MCF-7, HepG2, human kidney tissue, human small intestine tissue, mouse kidney tissue, human testis tissue.

Predicted band size: 92 kDa

Cell membrane, Cell projection, Cytoplasm, Membrane, Secreted.

1 mg/mL.

WB: 1:1,000-1:5,000 ;IF-Cell: 1:100-1:500 ;IHC-P: 1:50-1:1,000 ;IP: Use at an assay dependent concentration.

ET1611-58_2.jpg

Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate Lane 2: Mouse testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 92 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-58) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

ET1611-58_3.jpg

Western blot analysis of ACE2 on Hamster testis (1) and stomach (2) tissue lysates using anti-ACE2 antibody at 1/1,000 dilution.

ET1611-58_4.jpg

ICC staining of ACE2 in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ET1611-58_5.jpg

ICC staining of ACE2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ET1611-58_6.jpg

ICC staining of ACE2 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ET1611-58_7.jpg

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1611-58_8.jpg

Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-ACE2 antibody (ET1611-58) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-58) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1611-58_9.jpg

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ACE2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-58, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1611-58_10.jpg

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.