antibody

Anti-Integrin alpha 2 Antibody [SN0752]

ET1611-57TR

20 uL

99 USD

monoclonal

domestic rabbit

nonconjugated

SN0752

human, mouse, rat

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Anti-Integrin alpha 2 Antibody [SN0752]

Human,Mouse,Rat

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Non-conjugated

Synthetic peptide within Human Integrin alpha 2 aa 1,132-1,181 / 1,181.

SwissProt: P17301 Human;SwissProt: Q62469 Mouse;Unigene:83597 Rat

Rabbit

SN0752

IgG

20 uL

99 USD

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Liquid

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Protein A affinity purified.

Recombinant Rabbit monoclonal Antibody

MCF7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, human colon carcinoma tissue, human breast carcinoma tissue, human kidney tissue, mouse colon tissue, mouse stomach tissue, A431.

Predicted band size: 129 kDa

Membrane

1 mg/mL.

WB: 1:5,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

ET1611-57_2.jpg

Western blot analysis of Integrin alpha 2 on different lysates with Rabbit anti-Integrin alpha 2 antibody (ET1611-57) at 1/5,000 dilution. Lane 1: MCF7 cell lysate Lane 2: Mouse spleen tissue lysate Lane 3: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 129 kDa Observed band size: 150 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-57) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

ET1611-57_3.jpg

Integrin alpha 2 was immunoprecipitated in 0.2mg MCF7 cell lysate with ET1611-57 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET1611-57 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: MCF7 cell lysate (input) Lane 2: Rabbit IgG instead of ET1611-57 in MCF7 cell lysate Lane 3: ET1611-57 IP in MCF7 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds

ET1611-57_4.jpg

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Integrin alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1611-57_5.jpg

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Integrin alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1611-57_6.jpg

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Integrin alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1611-57_7.jpg

Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Integrin alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1611-57_8.jpg

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Integrin alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1611-57_9.jpg

Flow cytometric analysis of Integrin alpha 2 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-57, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).