Phospho-Cdk2 (Y15) | HUABIO ET1611-52 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-Cdk2 (Y15)

catalog :

ET1611-52

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN72-04

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-Cdk2 (Y15) on different lysates with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/1,000 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Lane 3: Mouse spleen tissue lysate (20 µg/Lane) Predicted band size: 34 kDa Observed band size: 35 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-52) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Phospho-Cdk2(Y15) on Hela cell lysates. Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Cdk2(Y15) antibody (ET1611-52) at 1/500 dilution. Anti-Cdk2 antibody (ET1602-6) at 1/500 dilution. Anti-GAPDH antibody (ET1601-4) at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 34 kDa Observed band size: 34 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds

image 3 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-52) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1611-52

Target name :

Phospho-Cdk2 (Y15)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Tyr15 of human Cdk2.

Uniprot id :

P24941>SwissProt: P24941 Human;SwissProt: P97377 Mouse;SwissProt: Q63699 Rat

Host :

Rabbit

Clone number :

SN72-04

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Hela cell lysate, NIH/3T3 cell lysate, mouse spleen tissue lysate, human breast carcinoma tissue, mouse small intestine tissue.

Molecular wt :

Predicted band size: 34 kDa

Subcellular location :

Cytoplasm, Cytoskeleton, Endosome, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200-1:1,000 ;IP: Use at an assay dependent concentration. ;IF-Tissue:1:50-1:200

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1611-52_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-52) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1611-52_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Phospho-Cdk2 (Y15) antibody (ET1611-52) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-52) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage