product summary
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company name :
HUABIO
product type :
antibody
product name :
Histone H2B (acetyl K20)
catalog :
ET1611-48
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
SN72-09
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
ICC staining Histone H2B(acetyl K20) (1/500) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
image 2 :
ICC staining Histone H2B(acetyl K20) (1/50) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
image 3 :
ICC staining Histone H2B(acetyl K20) (1/200) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
product information
SKU :
ET1611-48
Target name :
Histone H2B (acetyl K20)
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IF-Tissue,IHC-P,FC,IP,ChIP,CUT&Tag-seq
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human Histone H2B aa 1-49/126.
Uniprot id :
O60814>SwissProt: O60814 Human;SwissProt: P06899 Human;SwissProt: P23527 Human;SwissProt: P33778 Human;SwissProt: P57053 Human;SwissProt: P58876 Human;SwissProt: P62807 Human;SwissProt: Q16778 Human;SwissProt: Q5QNW6 Human;SwissProt: Q8N257 Human;SwissProt: Q93079 Human;SwissProt: Q99877 Human;SwissProt: Q99879 Human;SwissProt: Q99880 Human;Entrez Gene: 64647 Mouse;Entrez Gene: Q00729 Rat
Host :
Rabbit
Clone number :
SN72-09
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SW480, MCF-7, Hela, human tonsil tissue, human kidney tissue, human colon tissue, mouse colon tissue, human colon cancer tissue, mouse kidney tissue, human breast carcinoma tissue.
Molecular wt :
Predicted band size: 14 kDa
Subcellular location :
Chromosome, Nucleosome core, Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IF-Cell: 1:100-1:500
;IF-Tissue: 1:100-1:500
;IHC-P: 1:100-1:500
;FC: 1:50-1:100
;IP: Use at an assay dependent concentration.
;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
Advanced Validation :
Cell treatment (CT)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET1611-48_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET1611-48_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET1611-48_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/ET1611-48_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/ET1611-48_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/ET1611-48_9.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.
Pic img10 :
https://storage.huabio.cn/huabio/productImg/ET1611-48_10.jpg
Pic legend10 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.
Pic img11 :
https://storage.huabio.cn/huabio/productImg/ET1611-48_11.jpg
Pic legend11 :
Flow cytometric analysis of Hela cells with Histone H2B(acetyl K20) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was use
Pic img12 :
https://storage.huabio.cn/huabio/productImg/ET1611-48_12.jpg
Pic legend12 :
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 500ng/mL TSA for 4 hours with Histone H2B (acetyl K20) (ET1611-48) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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