product summary
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company name :
HUABIO
product type :
antibody
product name :
NEDD4L
catalog :
ET1611-42
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
SN73-03
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
more info or order :
image
image 1 :
Western blot analysis of NEDD4L on different lysates with Rabbit anti-NEDD4L antibody (ET1611-42) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: HT-29 cell lysate (20 µg/Lane)
Lane 3: HCT 116 cell lysate (20 µg/Lane)
Lane 4: SW480 cell lysate (20 µg/Lane)
Predicted band size: 112 kDa
Observed band size: 130 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of NEDD4L on different lysates with Rabbit anti-NEDD4L antibody (ET1611-42) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate (15 µg/Lane)
Lane 2: C2C12 cell lysate (15 µg/Lane)
Lane 3: C6 cell lysate (15 µg/Lane)
Lane 4: L6 cell lysate (15 µg/Lane)
Predicted band size: 112 kDa
Observed band size: 130 kDa
Exposure time: 17 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
NEDD4L was immunoprecipitated from 0.2 mg Jurkat cell lysate with ET1611-42 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1611-42 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (input)
Lane 2: ET1611-42 IP in Jurkat cell lysate
Lane 3: Rabbit IgG instead of ET1611-42 in Jurkat cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 minutes; ECL: K1801
product information
SKU :
ET1611-42
Target name :
NEDD4L
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human NEDD4L aa 926-975 / 975.
Uniprot id :
Q96PU5>SwissProt: Q96PU5 Human;SwissProt: Q8CFI0 Mouse;Unigene:72824 Rat
Host :
Rabbit
Clone number :
SN73-03
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Jurkat cell lysate, HT-29 cell lysate, HCT 116 cell lysate, SW480 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, L6 cell lysate.
Molecular wt :
Predicted band size: 112 kDa
Subcellular location :
Cytoplasm, Endosome, Golgi apparatus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:5,000
;IP: 1-2μg/sample
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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