NSD3 | HUABIO ET1611-39 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NSD3

catalog :

ET1611-39

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN07-11

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NSD3 on different lysates with Rabbit anti-NSD3 antibody (ET1611-39) at 1/10,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Rat brain tissue lysate Lane 5: Rat eyeball tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 162 kDa Observed band size: 162 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-39) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-NSD3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-39, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-NSD3 antibody (ET1611-39) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-39) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1611-39

Target name :

NSD3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,FC,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human NSD3 aa 1-50 / 1,437.

Uniprot id :

Q9BZ95>SwissProt: Q9BZ95 Human;SwissProt: Q6P2L6 Mouse;SwissProt: D3ZK47

Host :

Rabbit

Clone number :

SN07-11

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, MCF7 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat eyeball tissue lysate, human small intestine tissue, human lymph nodes tissue, Hela.

Molecular wt :

Predicted band size: 162 kDa

Subcellular location :

Nucleus, Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:10,000 ;FC: 1:50-1:100 ;IHC-P: 1:50-1:400

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1611-39_4.jpg

Pic legend4 :

Flow cytometric analysis of NSD3 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-39, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage