MAP1LC3A | HUABIO ET1611-37 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MAP1LC3A

catalog :

ET1611-37

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN07-40

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MAP1LC3A on different lysates with Rabbit anti-MAP1LC3A antibody (ET1611-37) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50μM Chloroquine for 18 hours cell lysate Lane 3: C2C12 cell lysate Lane 4: C2C12 treated with 50μM Chloroquine for 18 hours cell lysate Lane 5: C6 cell lysate Lane 6: C6 treated with 50μM Chloroquine for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 14/16 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-37) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MAP1LC3A antibody (ET1611-37) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-37) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MAP1LC3A antibody (ET1611-37) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-37) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1611-37

Target name :

MAP1LC3A

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein of Human MAP1LC3A aa 1-103 / 121.

Uniprot id :

Q9H492>SwissProt: Q9H492 Human;SwissProt: Q91VR7 Mouse;SwissProt: Q6XVN8 Rat

Host :

Rabbit

Clone number :

SN07-40

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HeLa treated with 50μM Chloroquine for 18 hours cell lysate, C2C12 cell lysate, C2C12 treated with 50μM Chloroquine for 18 hours cell lysate, C6 cell lysate, C6 treated with 50μM Chloroquine for 18 hours cell lysate, human brain tissue, rat brain tissue.

Molecular wt :

Predicted band size: 14 kDa

Subcellular location :

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:500-1:2,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage