Phospho-PDGFR beta (Y740) | HUABIO ET1611-29 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-PDGFR beta (Y740)

catalog :

ET1611-29

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN0646

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-PDGFR beta (Y740) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y740) antibody (ET1611-29) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 50 ng/mL PDGF for 5 minutes whole cell lysate Lane 3: NIH/3T3 treated with λpp whole cell lysate Lane 4: NIH/3T3 treated with 50 ng/mL PDGF for 5 minutes then treated with λpp whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 124 kDa Observed band size: 180 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-29) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining PDGF Receptor beta(phospho Y740) in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining PDGF Receptor beta(phospho Y740) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ET1611-29

Target name :

Phospho-PDGFR beta (Y740)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Tyr740 of human PDGFR beta.

Uniprot id :

P09619>SwissProt: P09619 Human;SwissProt: P05622 Mouse;SwissProt: Q05030 Rat

Host :

Rabbit

Clone number :

SN0646

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

NIH/3T3 treated with 50 ng/mL PDGF for 5 minutes whole cell lysate, C2C12, Hela, L6.

Molecular wt :

Predicted band size: 124 kDa

Subcellular location :

Cell membrane, Cytoplasmic vesicle.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1611-29_4.jpg

Pic legend4 :

ICC staining PDGF Receptor beta(phospho Y740) in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

more info or order :

HUABIO product webpage