Phospho-SHP2 (Y542) | HUABIO ET1611-22 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-SHP2 (Y542)

catalog :

ET1611-22

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN61-01

reactivity :

human, mouse

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-SHP2 (Y542) on different lysates with Rabbit anti-Phospho-SHP2 (Y542) antibody (ET1611-22) at 1/2,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 40ng/mL PDGF for 40 minutes whole cell lysate Lane 3: NIH/3T3 treated with 40ng/mL PDGF for 40 minutes then treated with λpp for 1 hour whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-22) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1611-22

Target name :

Phospho-SHP2 (Y542)

Species reactivity :

Mouse,Human

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Tyr542 of Human SHP2 aa 511-560 / 593.

Uniprot id :

Q06124>SwissProt: Q06124 Human;SwissProt: P35235 Mouse

Host :

Rabbit

Clone number :

SN61-01

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

NIH/3T3 treated with 40ng/ml PDGF for 40 minutes whole cell lysate.

Molecular wt :

Predicted band size: 68 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage