product summary
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company name :
HUABIO
product type :
antibody
product name :
hnRNP C1+C2
catalog :
ET1611-2
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
SN0652
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of hnRNP C1+C2 on different lysates with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/2,000 dilution.
Lane 1: MDA-MB-231 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: HepG2 cell lysate (10 µg/Lane)
Lane 4: MCF7 cell lysate (10 µg/Lane)
Lane 5: HL-60 cell lysate (10 µg/Lane)
Lane 6: Mouse brain tissue lysate (20 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 43 kDa
Exposure time: Lane 1-5: 10 seconds; Lane 6: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-2) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of hnRNP C1+C2 on different lysates with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: PC-12 cell lysate (20 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 43 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-2) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-2) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
ET1611-2
Target name :
hnRNP C1+C2
Species reactivity :
Human,Mouse,Rat,Zebrafish
Applications :
WB,IF-Tissue,IHC-P,IF-Cell,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein of Human HNRNPC aa 60-190 / 306.
Uniprot id :
P07910>SwissProt: P07910 Human;SwissProt: Q9Z204 Mouse;SwissProt: P17132 Rat
Host :
Rabbit
Clone number :
SN0652
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
MDA-MB-231 cell lysate, HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, HL-60 cell lysate, mouse brain tissue lysate, NIH/3T3 cell lysate, PC-12 cell lysate, rat brain tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, Hela, MCF-7, B16F1.
Molecular wt :
Predicted band size: 34 kDa
Subcellular location :
Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000-1:5,000
;IF-Tissue: 1:100-1:500
;IHC-P: 1:3,000-8,000
;IF-Cell: 1:100-1:500
;FC: 1:1,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET1611-2_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/8,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-2) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET1611-2_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/8,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-2) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET1611-2_6.jpg
Pic legend6 :
ICC staining hnRNP C1+C2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/ET1611-2_7.jpg
Pic legend7 :
ICC staining hnRNP C1+C2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/ET1611-2_8.jpg
Pic legend8 :
ICC staining hnRNP C1+C2 in B16F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/ET1611-2_9.jpg
Pic legend9 :
Flow cytometric analysis of HeLa cells labeling hnRNP C1+C2.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1611-2, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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