Phospho-Hormone sensitive lipase (S853) | HUABIO ET1611-19 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-Hormone sensitive lipase (S853)

catalog :

ET1611-19

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN06-39

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-Hormone sensitive lipase (S853) on different lysates with Rabbit anti-Phospho-Hormone sensitive lipase (S853) antibody (ET1611-19) at 1/1,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Rat skeletal muscle tissue lysate Lane 3: Rat skeletal muscle tissue lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 30 µg/Lane. Predicted band size: 117 kDa Observed band size: 84 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-19) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue with Rabbit anti-Phospho-Hormone sensitive lipase (S853) antibody (ET1611-19) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-19) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Phospho-Hormone sensitive lipase (S853) antibody (ET1611-19) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-19) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1611-19

Target name :

Phospho-Hormone sensitive lipase (S853)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser853 of Human LIPE aa 821-870 / 1,076.

Uniprot id :

Q05469>SwissProt: Q05469 Human;SwissProt: P54310-2 Mouse;SwissProt: P15304-2 Rat

Host :

Rabbit

Clone number :

SN06-39

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, human fetal skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue.

Molecular wt :

Predicted band size: 117 kDa

Subcellular location :

Cell membrane, cytosol, caveola, Lipid droplet.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1611-19_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Phospho-Hormone sensitive lipase (S853) antibody (ET1611-19) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-19) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage