Phospho-RPA32/RPA2 (T21) | HUABIO ET1611-18 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-RPA32/RPA2 (T21)

catalog :

ET1611-18

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN06-36

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-RPA32/RPA2 (T21) on different lysates with Rabbit anti-Phospho-RPA32/RPA2 (T21) antibody (ET1611-18) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 1uM Camptothecin for 1 hour whole cell lysate Lane 3: HEK-293 whole cell lysate Lane 4: HEK-293 treated with UV for 20 minutes, then recover 2 hours whole cell lysate Lane 5: HEK-293 treated with UV for 40 minutes, then recover 2 hours whole cell lysate Lane 6: HeLa whole cell lysate Lane 7: HeLa treated with UV for 40 minutes, then recover 2 hours whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29/35 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-18) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells treated with or without UV for 40 minutes then recover 2 hours labeling Phospho-RPA32/RPA2 (T21) with Rabbit anti-Phospho-RPA32/RPA2 (T21) antibody (ET1611-18) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-RPA32/RPA2 (T21) antibody (ET1611-18) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Western blot analysis of Phospho-RPA32/RPA2 (T21) on different lysates with Rabbit anti-Phospho-RPA32/RPA2 (T21) antibody (ET1611-18) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa (unphosphorylated) Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-18) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1611-18

Target name :

Phospho-RPA32/RPA2 (T21)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Thr21 of Human RPA32 / RPA2 aa 1-50 / 270.

Uniprot id :

P15927>SwissProt: P15927 Human;SwissProt: Q62193 Mouse;SwissProt: Q63528 Rat

Host :

Rabbit

Clone number :

SN06-36

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa whole cell lysate, HeLa treated with 1uM Camptothecin for 1 hour whole cell lysate, HEK-293 whole cell lysate, HEK-293 treated with UV for 20 minutes then recover 2 hours whole cell lysate, HEK-293 treated with UV for 40 minutes then recover 2 hours whole cell lysate, HeLa treated with UV for 40 minutes then recover 2 hours whole cell lysate, HeLa, Hela cell lysate, HL-60 cell lysate.

Molecular wt :

Predicted band size: 29 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IP: Use at an assay dependent concentration. ;IF-Cell: 1:50

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage