Phospho-MSK1 (S376) | HUABIO ET1611-17 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-MSK1 (S376)

catalog :

ET1611-17

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SN63-05

reactivity :

human

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of HEK-293 cells treated with or without 200nM PMA for 30 minutes labeling Phospho-MSK1 (S376) with Rabbit anti-Phospho-MSK1 (S376) antibody (ET1611-17) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-MSK1 (S376) antibody (ET1611-17) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Phospho-MSK1 (S376) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-Phospho-MSK1 (S376) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-17, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1611-17

Target name :

Phospho-MSK1 (S376)

Species reactivity :

Human,Rat

Applications :

WB,IF-Cell,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser376 of Human MSK1 aa 351-400 / 802.

Uniprot id :

O75582>SwissProt: O75582 Human;Entrez Gene: 314384 Rat

Host :

Rabbit

Clone number :

SN63-05

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HEK-293 cells treated with 200nM PMA for 30 minutes, human colon tissue, rat bladder tissue.

Molecular wt :

Predicted band size: 90 kDa

Subcellular location :

Nucleus, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage