Anti-Caspase-9 Antibody [SC65-05] | HUABIO ET1610-95TR product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Anti-Caspase-9 Antibody [SC65-05]

catalog :

ET1610-95TR

quantity :

20 uL

price :

99 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SC65-05

reactivity :

human, mouse

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

product information

SKU :

ET1610-95TR

Target name :

Anti-Caspase-9 Antibody [SC65-05]

Species reactivity :

Human,Mouse

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Caspase-9 aa 95-335 / 416.

Uniprot id :

SwissProt: P55211 Human;SwissProt: Q8C3Q9 Mouse

Host :

Rabbit

Clone number :

SC65-05

Isotype :

IgG

Size :

20 uL

List Price :

99 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

C2C12 cell lysate, Hela cell lysate, Jurkat cell lysate.

Molecular wt :

Predicted band size: 46/30/17/37 kDa

Subcellular location :

Apoptosome, cytosol, Mitochondrion, nucleus, cytoplasm, protein-containing complex.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IP: Use at an assay dependent concentration.

Pic img4 :

ET1610-95_2.jpg

Pic img5 :

Western blot analysis of Caspase-9 on different lysates with Rabbit anti-Caspase-9 antibody (ET1610-95) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si Caspase-9 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. ET1610-95 was shown to specifically react with Caspase-9 in Hela-si NT cells. Weakened band was observed when Hela-si Caspase-9 sample was tested. Hela-si NT and Hela-si Caspase-9 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-95, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage