GAP43 | HUABIO ET1610-94-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GAP43

catalog :

ET1610-94-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SC60-06

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GAP43 on different lysates with Rabbit anti-GAP43 antibody (ET1610-94) at 1/100,000 dilution and competitor's antibody at 1/100,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: A549 cell lysate (negative) Lane 3: Neuro-2a cell lysate Lane 4: Mouse lung tissue lysate (negative) Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 43/45 kDa Exposure time: 3 minutes 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-94) at 1/100,000 dilution and competitor's antibody at 1/100,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human glioma tissue with Rabbit anti-GAP43 antibody (ET1610-94) at 1/10,000 dilution and competitor's antibody at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-94) at 1/10,000 dilution and competitor's antibody at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunocytochemistry analysis of Neuro-2a cells labeling GAP43 with Rabbit anti-GAP43 antibody (ET1610-94) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GAP43 antibody (ET1610-94) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

ET1610-94-50UL

Target name :

GAP43

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human GAP43 aa 189-238 / 238.

Uniprot id :

P17677>SwissProt: P17677 Human;SwissProt: P06837 Mouse;SwissProt: P07936 Rat

Host :

Rabbit

Clone number :

SC60-06

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SH-SY5Y cell lysate, Neuro-2a cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human glioma tissue, Neuro-2a, rat hippocampus tissue lysate, mouse hippocampus tissue lysate, mouse brain tissue, rat brain tissue, rat hippocampus tissue, SH-SY5Y.

Molecular wt :

Predicted band size: 25 kDa

Subcellular location :

Cell membrane, growth cone membrane, filopodium membrane, Cytoplasm, synapse, perikaryon, dendrite, axon.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:20,000-1:100,000 ;IF-Cell: 1:500 ;IF-Tissue: 1:500-1:1,000 ;IHC-P: 1:10,000-1:15,000 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Relative expression (RE),Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1610-94_4.jpg

Pic legend4 :

Western blot analysis of GAP43 on different lysates with Rabbit anti-GAP43 antibody (ET1610-94) at 1/20,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Neuro-2a cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Rat brain tissue lysate Lane 5: Rat hippocampus tissue lysate Lane 6: Mouse hippocampus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 43/45 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-94) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1610-94_5.jpg

Pic legend5 :

Western blot analysis of GAP43 on different lysates with Rabbit anti-GAP43 antibody (ET1610-94) at 1/20,000 dilution. Lane 1: SH-SY5Y-si NT cell lysate Lane 2: SH-SY5Y-si GAP43 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 25 kDa Observed band size: 45 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-94) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1610-94_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GAP43 antibody (ET1610-94) at 1/15,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-94) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1610-94_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GAP43 antibody (ET1610-94) at 1/15,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-94) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1610-94_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GAP43 antibody (ET1610-94) at 1/15,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-94) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1610-94_9.jpg

Pic legend9 :

Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling GAP43 with Rabbit anti-GAP43 antibody (ET1610-94) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1610-94, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1610-94_10.jpg

Pic legend10 :

Intracellular Flow Cytometry analysis of SH-SY5Y labeling GAP43 with purified ET1610-94 at 1/1,000 dilution (1 µg/ml) (red). Cells were fixed with 4% PFA and permeabilised with 90% methanol. Rabbit monoclonal IgG (green) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (black) were used as the unlabeled control. A Goat anti-rabbit IgG iFluor™ 488 (HA1121)(1/1,000 dilution) was used as the secondary antibody.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET1610-94_11.jpg

Pic legend11 :

Flow cytometric analysis of Neuro-2a cells labeling GAP43. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1610-94, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage