C/EBP Beta | HUABIO ET1610-9-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

C/EBP Beta

catalog :

ET1610-9-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SC55-05

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Flow cytometric analysis of C/EBP Beta was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-9, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

image 2 :

Western blot analysis of C/EBP Beta on different lysates with Rabbit anti-C/EBP Beta antibody (ET1610-9) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-87 MG cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 60 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-9) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1610-9-50UL

Target name :

C/EBP Beta

Species reactivity :

Human,Rat,Mouse

Applications :

WB,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Rat C/EBP Beta aa 248-297 / 297.

Uniprot id :

P17676>SwissProt: P17676 Human;SwissProt: P28033 Mouse;SwissProt: P21272 Rat

Host :

Rabbit

Clone number :

SC55-05

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

PC-12 cell lysates, Hela, MCF-7, PC-12.

Molecular wt :

Predicted band size: 36 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

more info or order :

HUABIO product webpage