product summary
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company name :
HUABIO
product type :
antibody
product name :
Sonic Hedgehog Protein/SHH(C-Product)
catalog :
ET1610-6
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
SC05-28
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Sonic Hedgehog Protein/SHH(C-Product) on different lysates with Rabbit anti-Sonic Hedgehog Protein/SHH(C-Product) antibody (ET1610-6) at 1/1,000 dilution.
Lane 1: Mouse kidney tissue lysate (30 µg/Lane)
Lane 2: Mouse brain tissue lysate (30 µg/Lane)
Lane 3: Mouse liver tissue lysate (30 µg/Lane)
Lane 4: H22 cell lysate (15 µg/Lane)
Lane 5: Neuro-2a cell lysate (15 µg/Lane)
Lane 6: NIH/3T3 cell lysate (15 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 28/55 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-6) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Sonic Hedgehog Protein/SHH(C-Product) on different lysates with Rabbit anti-Sonic Hedgehog Protein/SHH(C-Product) antibody (ET1610-6) at 1/1,000 dilution.
Lane 1: Rat heart tissue lysate
Lane 2: Rat liver tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat lung tissue lysate
Lane 5: Mouse liver tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 28/55 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-6) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Western blot analysis of Sonic Hedgehog Protein on different lysates using anti-Sonic Hedgehog Protein antibody at 1/1,000 dilution.
Positive control: Lane 1: Hela Lane 2: HepG2
product information
SKU :
ET1610-6
Target name :
Sonic Hedgehog Protein/SHH(C-Product)
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IF-Tissue,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human Sonic Hedgehog Protein aa 258-307 / 462.
Uniprot id :
Q15465>SwissProt: Q15465 Human;SwissProt: Q62226 Mouse;SwissProt: Q63673 Rat
Host :
Rabbit
Clone number :
SC05-28
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Mouse kidney tissue lysate, Mouse brain tissue lysate, Mouse liver tissue lysate, H22 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, Rat heart tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, Rat lung tissue lysate, Neuro-2a, Hela, human liver tissue, mouse liver tissue, rat kidney tissue, rat liver tissue.
Molecular wt :
Predicted band size: 50 kDa
Subcellular location :
Secreted, Cell membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
;IF-Cell: 1:50-1:200
;IF-Tissue: 1:50-1:200
;IHC-P: 1:200-1:1,000
;FC: 1:50-1:100
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET1610-6_4.jpg
Pic legend4 :
Immunocytochemistry analysis of Neuro-2a cells labeling Sonic Hedgehog Protein/SHH(C-Product) with Rabbit anti-Sonic Hedgehog Protein/SHH(C-Product) antibody (ET1610-6) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Sonic Hedgehog Protein/SHH(C-Product) antibody (ET1610-6) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET1610-6_5.jpg
Pic legend5 :
ICC staining Sonic Hedgehog Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET1610-6_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Sonic Hedgehog Protein/SHH(C-Product) antibody (ET1610-6) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-6) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/ET1610-6_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Sonic Hedgehog Protein/SHH(C-Product) antibody (ET1610-6) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-6) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/ET1610-6_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/ET1610-6_9.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.
Pic img10 :
https://storage.huabio.cn/huabio/productImg/ET1610-6_10.jpg
Pic legend10 :
Flow cytometric analysis of Hela cells with Sonic Hedgehog Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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