antibody

Anti-Wilms Tumor Protein Antibody [SC06-41]

ET1610-45TR

20 uL

99 USD

monoclonal

domestic rabbit

nonconjugated

SC06-41

human, mouse

western blot, flow cytometry, immunohistochemistry - paraffin section

Anti-Wilms Tumor Protein Antibody [SC06-41]

Human,Mouse

WB,IF-Cell,IF-Tissue,IHC-P,FC

Non-conjugated

Recombinant protein within Human WT1 aa 59-269 / 449.

SwissProt: P19544 Human;SwissProt: P22561 Mouse

Rabbit

SC06-41

IgG

20 uL

99 USD

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Liquid

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Protein A affinity purified.

Recombinant Rabbit monoclonal Antibody

K-562 cell lysates, Hela, LO2, PC-3M, mouse kidney tissue, K562, human fallopian tube tissue, human testis tissue, human ovary cancer tissue.

Predicted band size: 55 kDa

Nucleus, Cytoplasm, Nucleus speckle.

1 mg/mL.

WB: 1:2,000-1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:500 ;FC: 1:50-1:100

ET1610-45_2.jpg

ICC staining of Wilms Tumor Protein in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ET1610-45_3.jpg

ICC staining of Wilms Tumor Protein in LO2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ET1610-45_4.jpg

ICC staining of Wilms Tumor Protein in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ET1610-45_5.jpg

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Wilms Tumor Protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-45, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1610-45_6.jpg

Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-Wilms Tumor Protein antibody (ET1610-45) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-45) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1610-45_7.jpg

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Wilms Tumor Protein antibody (ET1610-45) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-45) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1610-45_8.jpg

Flow cytometric analysis of Wilms Tumor Protein was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-45, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

ET1610-45_9.jpg

Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-Wilms Tumor Protein antibody (ET1610-45) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-45) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.