Phospho-EGFR (Y1173) | HUABIO ET1610-4 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-EGFR (Y1173)

catalog :

ET1610-4

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SC57-04

reactivity :

human, mouse

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-EGFR (Y1173) on different lysates with Rabbit anti-Phospho-EGFR (Y1173) antibody (ET1610-4) at 1/2,000 dilution. Lane 1: A431 whole cell lysate Lane 2: A431 treated with 100ng/mL EGF for 30 minutes whole cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 135 kDa Observed band size: 170 kDa Exposure time: 2 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-4) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of A431 cells treated with or without 100ng/mL EGF for 5 minutes labeling Phospho-EGFR (Y1173) with Rabbit anti-Phospho-EGFR (Y1173) antibody (ET1610-4) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at 37 ℃, permeabilized with 0.1% Triton X-100 in PBS permeabilization for 5 minutes, and then blocked with 2% negative goat serum for 60 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-EGFR (Y1173) antibody (ET1610-4) at 1/50 dilution in 1% BSA overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 3 :

ICC staining of Phospho-EGFR (Y1173) in untreated B16F1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

ET1610-4

Target name :

Phospho-EGFR (Y1173)

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Tyr1173 of Human EGFR.

Uniprot id :

P00533>SwissProt: P00533 Human;SwissProt: Q01279 Mouse

Host :

Rabbit

Clone number :

SC57-04

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 treated with 100ng/mL EGF for 30 minutes whole cell lysate, A431, B16F1, human placenta tissue, mouse skin tissue.

Molecular wt :

Predicted band size: 134 kDa

Subcellular location :

Cell membrane, Nucleus membrane, Nucleus, Endoplasmic reticulum membrane, Golgi apparatus membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1610-4_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Phospho-EGFR (Y1173) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-4, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1610-4_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Phospho-EGFR (Y1173) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-4, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage