PI3-kinase p110 subunit beta | HUABIO ET1610-36 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PI3-kinase p110 subunit beta

catalog :

ET1610-36

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SC0580

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PI3-kinase p110 subunit beta on different lysates with Rabbit anti-PI3-kinase p110 subunit beta antibody (ET1610-36) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 123 kDa Observed band size: 110 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-36) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PI3-kinase p110 subunit beta on different lysates with Rabbit anti-PI3-kinase p110 subunit beta antibody (ET1610-36) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PI3-kinase p110 subunit beta KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 123 kDa Observed band size: 110 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-36) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-PI3-kinase p110 subunit beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1610-36

Target name :

PI3-kinase p110 subunit beta

Species reactivity :

Human

Applications :

WB,IP,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human PIK3CB aa 5-52 / 1070.

Uniprot id :

P42338>SwissProt: P42338 Human

Host :

Rabbit

Clone number :

SC0580

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, K-562 cell lysate, HeLa cell lysate, MCF7 cell lysate, human liver carcinoma tissue, Hela.

Molecular wt :

Predicted band size: 123 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:50-1:100 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1610-36_4.jpg

Pic legend4 :

Flow cytometric analysis of PI3-kinase p110 subunit beta was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-36, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage