Anti-Integrin alpha V Antibody [SC56-07] | HUABIO ET1610-15TR product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Anti-Integrin alpha V Antibody [SC56-07]

catalog :

ET1610-15TR

quantity :

20 uL

price :

99 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SC56-07

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

product information

SKU :

ET1610-15TR

Target name :

Anti-Integrin alpha V Antibody [SC56-07]

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,FC,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within of Human Integrin alpha V aa 1000-1048 / 1048.

Uniprot id :

SwissProt: P06756 Human;SwissProt: P43406 Mouse;Entrez Gene: 296456 Rat

Host :

Rabbit

Clone number :

SC56-07

Isotype :

IgG

Size :

20 uL

List Price :

99 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A549 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, rat heart tissue lysate, A549, SiHa, MCF-7, human placenta tissue.

Molecular wt :

Predicted band size: 116 kDa

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:5,000 ;IF-Cell: 1:50-1:100 ;FC: 1:50-1:100 ;IHC-P: 1:1,000

Pic img4 :

ET1610-15_2.jpg

Pic img5 :

All lanes: Western blot analysis of Integrin alpha V with anti-Integrin alpha V antibody (ET1610-15) at 1/500 dilution. Lane 1: Wild-type A549 whole cell lysate. Lane 2/3: Integrin alpha V knockdown A549 whole cell lysate. ET1610-15 was shown to specifically react with Integrin alpha V in Wild-type A549 cells. Weakened bands were observed when Integrin alpha V knockdown samples were tested. Wild-type and Integrin alpha V knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Integrin alpha V antibody (ET1610-15, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Pic legend5 :

ET1610-15_3.jpg

Pic legend6 :

Western blot analysis of Integrin alpha V on different lysates with Rabbit anti-Integrin alpha V antibody (ET1610-15) at 1/5,000 dilution. Lane 1: A549 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 116 kDa Observed band size: 130 kDa Exposure time: 4 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-15) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Pic img7 :

ET1610-15_4.jpg

Pic img8 :

ICC staining of Integrin alpha V in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic legend8 :

ET1610-15_5.jpg

Pic legend9 :

ICC staining of Integrin alpha V in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img10 :

ET1610-15_6.jpg

Pic img11 :

Flow cytometric analysis of Integrin alpha V was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-15, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic legend11 :

ET1610-15_7.jpg

Pic legend12 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Integrin alpha V antibody (ET1610-15) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage