Estrogen Related Receptor alpha | HUABIO ET1610-14 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Estrogen Related Receptor alpha

catalog :

ET1610-14

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SC57-07

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Estrogen Related Receptor alpha on different lysates with Rabbit anti-Estrogen Related Receptor alpha antibody (ET1610-14) at 1/2,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: mouse brain tissue lysate Lane 3: mouse kidney tissue lysate Lane 4: rat brain tissue lysate Lane 5: rat kidney tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of Estrogen Related Receptor alpha was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-14, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

image 3 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Estrogen Related Receptor alpha antibody (ET1610-14) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-14) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1610-14

Target name :

Estrogen Related Receptor alpha

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP,IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Estrogen Related Receptor alpha aa 1-49 / 423.

Uniprot id :

P11474>SwissProt: P11474 Human;SwissProt: O08580 Mouse;SwissProt: Q5QJV7 Rat

Host :

Rabbit

Clone number :

SC57-07

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF-7 cell lysate, SK-Br-3 cell lysate, PC-3 cell lysate, human kidney tissue, mouse brain tissue, Hela.

Molecular wt :

Predicted band size: 46 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;FC: 1:50-1:100 ;IHC-P: 1:1,000 ;IF-Tissue: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1610-14_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Estrogen Related Receptor alpha antibody (ET1610-14) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-14) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1610-14_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Estrogen Related Receptor alpha antibody (ET1610-14) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-14) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1610-14_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Estrogen Related Receptor alpha antibody (ET1610-14) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-14) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1610-14_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Estrogen Related Receptor alpha antibody (ET1610-14) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-14) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1610-14_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Estrogen Related Receptor alpha antibody (ET1610-14) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-14) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1610-14_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Estrogen Related Receptor alpha antibody (ET1610-14) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-14) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage