Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) Antibody [SC58-01]

product summary

company name :

HUABIO

product type :

antibody

product name :

Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) Antibody [SC58-01]

catalog :

ET1610-13TR

quantity :

20 uL

price :

99 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SC58-01

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

product information

SKU :

ET1610-13TR

Target name :

Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) Antibody [SC58-01]

Species reactivity :

Human,Rat,Mouse

Applications :

WB,IP,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human aa 166-215 / 379.

Uniprot id :

SwissProt: P27361 Human;SwissProt: P28482 Human;SwissProt: P63085 Mouse;SwissProt: Q63844 Mouse;SwissProt: P21708 Rat;SwissProt: P63086 Rat

Host :

Rabbit

Clone number :

SC58-01

Isotype :

IgG

Size :

20 uL

List Price :

99 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SH-SY5Y cell lysate, SH-SY5Y treated with 100ng/mL hβ-NGF for 10 minutes cell lysate, PC-12 treated with 100ng/mL hβ-NGF for 10 minutes cell lysate, SK-Br-3 whole cell lysate, Jurkat treated with 200ng/ml PMA for 30 minutes cell lysates, human thyroid carcinoma tissue.

Molecular wt :

Predicted band size: 41/43 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IP: Use at an assay dependent concentration. ;IHC-P: 1:1,000

Pic img4 :

ET1610-13_2.jpg

Pic img5 :

Western blot analysis of Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Untreated SK-Br-3 whole cell lysates Lane 2: SK-Br-3 treated with λ Protein Phosphatase whole cell lysates

Pic legend5 :

ET1610-13_3.jpg

Pic legend6 :

Western blot analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates. Lane 1: Jurkat cells, whole cell lysate, 10ug/lane Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody (ET1610-13) at 1:500 dilution. Anti-ERK1+ERK2 antibody(ET1601-29) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size:41/43 kDa Observed band size:41/43 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 2 minutes 32 seconds.

Pic img7 :

ET1610-13_4.jpg

Pic img8 :

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (ET1610-13) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-13) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage