Histone H2B (mono methyl R79) | HUABIO ET1609-9 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Histone H2B (mono methyl R79)

catalog :

ET1609-9

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

ST0430

reactivity :

human, mouse, rat

application :

immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Histone H2B (mono methyl R79) antibody (ET1609-9) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-9) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Histone H2B (mono methyl R79) antibody (ET1609-9) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-9) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Histone H2B (mono methyl R79) antibody (ET1609-9) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-9) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1609-9

Target name :

Histone H2B (mono methyl R79)

Species reactivity :

Human,Mouse,Rat

Applications :

IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Histone H2B aa 51-100 / 126.

Uniprot id :

O60814>SwissProt: O60814 Human;SwissProt: P06899 Human;SwissProt: P23527 Human;SwissProt: P33778 Human;SwissProt: P57053 Human;SwissProt: P58876 Human;SwissProt: P62807 Human;SwissProt: Q16778 Human;SwissProt: Q5QNW6 Human;SwissProt: Q8N257 Human;SwissProt: Q93079 Human;SwissProt: Q99877 Human;SwissProt: Q99879 Human;SwissProt: Q99880 Human;SwissProt: P10854 Mouse;SwissProt: Q64475 Mouse;SwissProt: Q00729 Rat

Host :

Rabbit

Clone number :

ST0430

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse colon tissue, mouse testis tissue, rat colon tissue, rat testis tissue, human tonsil tissue, human breast carcinoma tissue, HepG2, Hela, MCF-7.

Molecular wt :

Predicted band size: 14 kDa

Subcellular location :

Nucleus, Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1609-9_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Histone H2B (mono methyl R79) antibody (ET1609-9) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-9) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1609-9_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1609-9_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1609-9_7.jpg

Pic legend7 :

ICC staining Histone H2B(mono methyl R79) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1609-9_8.jpg

Pic legend8 :

ICC staining Histone H2B(mono methyl R79) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1609-9_9.jpg

Pic legend9 :

ICC staining Histone H2B(mono methyl R79) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

more info or order :

HUABIO product webpage