Histone H3 (mono methyl R2) | HUABIO ET1609-8 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Histone H3 (mono methyl R2)

catalog :

ET1609-8

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

ST0427

reactivity :

human, mouse, rat

application :

western blot, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

ICC staining Histone H3(mono methyl R2) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 2 :

ICC staining Histone H3(mono methyl R2) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining Histone H3(mono methyl R2) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ET1609-8

Target name :

Histone H3 (mono methyl R2)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,ChIP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Histone H3 aa 1-49/136.

Uniprot id :

P68431>SwissProt: P68431 Human;SwissProt: P84243 Human;SwissProt: Q6NXT2 Human;SwissProt: Q71DI3 Human;SwissProt: Q16695 Human;SwissProt: P84228 Mouse;SwissProt: P68433 Mouse;SwissProt: Q6LED0 Rat

Host :

Rabbit

Clone number :

ST0427

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2, NIH/3T3, PC-3M, human skin tissue, human kidney tissue, mouse colon tissue, mouse kidney tissue, rat colon tissue, rat kidney tissue.

Molecular wt :

Predicted band size: 15 kDa

Subcellular location :

Nucleus, Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1609-8_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody (ET1609-8) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-8) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1609-8_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody (ET1609-8) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-8) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1609-8_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody (ET1609-8) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-8) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1609-8_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody (ET1609-8) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-8) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1609-8_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody (ET1609-8) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-8) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1609-8_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Histone H3 (mono methyl R2) antibody (ET1609-8) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-8) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1609-8_10.jpg

Pic legend10 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with Histone H3 (mono methyl R2) (ET1609-8) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

more info or order :

HUABIO product webpage