product summary
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company name :
HUABIO
product type :
antibody
product name :
Anti-COL1A1 Antibody [ST58-04]
catalog :
ET1609-68TR
quantity :
20 uL
price :
99 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
ST58-04
reactivity :
human, bovine
application :
western blot, immunohistochemistry - paraffin section
more info or order :
product information
SKU :
ET1609-68TR
Target name :
Anti-COL1A1 Antibody [ST58-04]
Species reactivity :
Human,Cow
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human COL1A1 aa 1191-1238 / 1464.
Uniprot id :
SwissProt: P02452 Human;Entrez Gene:282187 Cow
Host :
Rabbit
Clone number :
ST58-04
Isotype :
IgG
Size :
20 uL
List Price :
99 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Human placenta tissue lysates, human skin tissue lysates, human kidney tissue, human colon carcinoma tissue.
Molecular wt :
Predicted band size: 139 kDa
Subcellular location :
Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
;IHC-P: 1:1,000
Pic img4 :
ET1609-68_2.jpg
Pic img5 :
Western blot analysis of COL1A1 on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Pic legend5 :
ET1609-68_3.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-COL1A1 antibody (ET1609-68) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-68) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
ET1609-68_4.jpg
Pic img8 :
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-COL1A1 antibody (ET1609-68) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-68) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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