c-Kit | HUABIO ET1609-60 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

c-Kit

catalog :

ET1609-60

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

ST04-99

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of c-Kit on different lysates with Rabbit anti-c-Kit antibody (ET1609-60) at 1/2,000 dilution. Lane 1: Saos-2 cell lysate Lane 2: HL-60 cell lysate (low expression) Lane 3: Jurkat cell lysate (low expression) Lysates/proteins at 15 µg/Lane. Predicted band size: 110 kDa Observed band size: 110-140 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-60) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of c-Kit on different lysates with Rabbit anti-c-Kit antibody (ET1609-60) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-c-Kit KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 110 kDa Observed band size: 140 kDa Exposure time: 2 minute 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-60) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of c-Kit on human brain tissue lysates with Rabbit anti-c-Kit antibody (ET1609-60) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-60) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1609-60

Target name :

c-Kit

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminal human KIT.

Uniprot id :

P10721>SwissProt: P10721 Human

Host :

Rabbit

Clone number :

ST04-99

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human brain tissue lysates, Saos-2 cell lysate, human breast tissue, human gastrointestinal stromal tumor tissue.

Molecular wt :

Predicted band size: 110 kDa

Subcellular location :

Cell membrane, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:200

Advanced Validation :

Relative expression (RE),Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1609-60_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-c-Kit antibody (ET1609-60) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-60) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1609-60_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor tissue with Rabbit anti-c-Kit antibody (ET1609-60) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-60) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage