Phospho-BTK (Y223) | HUABIO ET1609-55 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-BTK (Y223)

catalog :

ET1609-55

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

ST51-05

reactivity :

human, mouse

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-BTK (Y223) on different lysates with Rabbit anti-Phospho-BTK (Y223) antibody (ET1609-55) at 1/1,000 dilution. Lane 1: Ramos cell lysate Lane 2: Ramos treated with 12μg/mL human IgM for 10 minutes cell lysate Lane 3: Ramos treated with 12μg/mL human IgM for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 76 kDa Observed band size: 76 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-55) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Phospho-BTK(Y223) on different lysates using anti-Phospho-BTK(Y223) antibody at 1/1,000 dilution. Positive control: Lane 1: K562 cells treated with pervanadate Lane 2: Untreated K562 cell lysate

product information

SKU :

ET1609-55

Target name :

Phospho-BTK (Y223)

Species reactivity :

Human,Mouse

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Tyr223 of human BTK.

Uniprot id :

Q06187>SwissProt: Q06187 Human;SwissProt: P35991 Mouse

Host :

Rabbit

Clone number :

ST51-05

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Ramos cell lysate, Ramos treated with 12μg/mL human IgM for 10 minutes cell lysate, K562 cells treated with pervanadate.

Molecular wt :

Predicted band size: 76 kDa

Subcellular location :

Cytoplasm, Nucleus, Cell membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage